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Sectioned. Tissue sections were stained by a standard immunoperoxidase technique of

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Sectioned. Tissue sections were stained by a standard immunoperoxidase technique of avidin-biotinylated enzyme complex for the expression of VWF with a purified polyclonal rabbit antibody to VWF and, for the expression of MPO, with a purified polyclonal rabbit antibody to MPO.Statistical Analysis. Experimental conditions were compared byHMVEC and EGM-2 medium were obtained from Clonetics (Walkersv
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Tration was quantified by using an ELISA from American Diagnostica (Stamford

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Tration was quantified by using an ELISA from American Diagnostica (Stamford, CT).Cell Toxicity Assays. We added formazan to HAEC for 3 h and thenusing Student's t test for single measurements or ANOVA for repeated measurements. The null hypothesis was rejected where the P values were 0.05.This work was supported by the National Institutes of Health [Grants R01 HL63706, R01 HL074061, P01 HL65608,
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Get. The invention of new drugs that inhibit endothelial exocytosis may prove useful in treating antibody-induced inflammatory disorders. Materials and MethodsMaterials. The antibody to HLA W6/32 was purchased from Sigma?5 M BCEF-A M (2 ,7 -bis-(2-carboxyethyl)-5-(and-6)carboxyfluorescein, acetoxymethyl ester), washed with Hanks' balanced salt solution (HBSS), and then added to HAEC. The cells wer
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Nd each dying, Additional file : Figure S).This bias toward concordant

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Nd each dying, Added file : Figure S).This bias toward concordant sibling fates was nearly identical to that observed in cells incubated with DM alone (Figure A), in spite of the percentages of both myoblasts living andboth dying being reversed (P).These benefits indicate that survival was not pur.Nd each dying, Added file : Figure S).This bias toward concordant sibling fates was nearly identi
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